TBAT Biology Special Test 3

A suspension of isolated mitochondria-oxidizing glutamate and malate in the presence of ADP is treated sequentially with: (1) oligomycin, (2) FCCP (ETC uncoupler), (3) rotenone, (4) ascorbate + TMPD (electron donor), and (5) potassium cyanide (KCN). What is the expected sequence of changes in oxygen consumption rate?

Fluoroacetate is metabolized in vivo to fluorocitrate. What is the metabolic consequence and direct target of fluorocitrate (an aconitase inhibitor) in the citric acid cycle?

Arsenite poisoning leads to a significant elevation in systemic levels of pyruvate, lactate, and $\alpha$-ketoglutarate. Which enzyme modification represents the shared metabolic target of arsenite?